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Isolation of human foetal myoblasts and its application for microencapsulation.
- Source :
- Journal of Cellular & Molecular Medicine; Jan/Feb2008, Vol. 12 Issue 1, p271-280, 10p, 3 Color Photographs, 2 Graphs
- Publication Year :
- 2008
-
Abstract
- Foetal cells secrete more growth factors, generate less immune response, grow and proliferate better than adult cells. These characteristics make them desirable for recombinant modification and use in microencapsulated cellular gene therapeutics. We have established a system in vitro to obtain a pure population of primary human foetal myoblasts under several rounds of selection with non-collagen coated plates and identified by desmin staining. These primary myoblasts presented good proliferation ability and better differentiation characteristics in monolayer and after microencapsulation compared to murine myoblast C2C12 cells based on creatine phosphokinase (CPK), major histocompatibility complex (MHC) and multi-nucleated myotubule determination. The lifespan of primary myoblasts was 70 population doublings before entering into senescent state, with a population time of 18-24 hrs. Hence, we have developed a protocol for isolating human foetal primary myoblasts with excellent differentiation potential and robust growth and longevity. They should be useful for cell-based therapy in human clinical applications with microencapsulation technology. [ABSTRACT FROM AUTHOR]
- Subjects :
- MYOBLASTS
MICROENCAPSULATION
GROWTH factors
IMMUNE response
COLLAGEN
MOUSE leukemia
Subjects
Details
- Language :
- English
- ISSN :
- 15821838
- Volume :
- 12
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Cellular & Molecular Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 38022224
- Full Text :
- https://doi.org/10.1111/j.1582-4934.2007.00119.x