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Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network.
- Source :
- Nature Methods; Jan2009, Vol. 6 Issue 1, p47-54, 8p, 2 Diagrams, 1 Chart, 2 Graphs
- Publication Year :
- 2009
-
Abstract
- To provide accurate biological hypotheses and elucidate global properties of cellular networks, systematic identification of protein-protein interactions must meet high quality standards. We present an expanded C. elegans protein-protein interaction network, or 'interactome' map, derived from testing a matrix of ∼10,000 × ∼10,000 proteins using a highly specific, high-throughput yeast two-hybrid system. Through a new empirical quality control framework, we show that the resulting data set (Worm Interactome 2007, or WI-2007) was similar in quality to low-throughput data curated from the literature. We filtered previous interaction data sets and integrated them with WI-2007 to generate a high-confidence consolidated map (Worm Interactome version 8, or WI8). This work allowed us to estimate the size of the worm interactome at ∼116,000 interactions. Comparison with other types of functional genomic data shows the complementarity of distinct experimental approaches in predicting different functional relationships between genes or proteins. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15487091
- Volume :
- 6
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Nature Methods
- Publication Type :
- Academic Journal
- Accession number :
- 37381407
- Full Text :
- https://doi.org/10.1038/nmeth.1279