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Effect of Progesterone on HLA-E Gene Expression in JEG-3 Choriocarcinoma Cell Line.
- Source :
- American Journal of Reproductive Immunology; Mar2009, Vol. 61 Issue 3, p221-226, 6p, 1 Chart, 2 Graphs
- Publication Year :
- 2009
-
Abstract
- Problem Among class Ib human leukocyte antigen (HLA) molecules, HLA-E is known to be a major ligand of CD94/NKG2 receptor on natural killer (NK) cells, and to play a pivotal role in recognition of extravillous trophoblasts (EVTs) by maternal immune cells. However, it is scarcely known how HLA-E expression is regulated in EVTs. Method of study In this study, we investigated whether progesterone, an essential hormone in maintaining pregnancy, regulated HLA-E expression in EVT-like cell line, JEG-3. HLA-E mRNA amount in cultured JEG-3 cells was assessed by real-time PCR and cell-surface HLA-E protein was analyzed by flowcytometry. Results Real-time PCR showed 3.5-fold increase 1 hour after the addition of 1000 ng/ml progesterone. This response was dimished by the addition of RU486, an antagonist for progesterone receptor. Flowcytometry indicated that 1000 ng/ml progesterone slightly enhanced HLA-E expression on the surface of JEG-3. Conclusion These results suggest that progesterone up-regulates HLA-E expression in JEG-3 cells through the pathway mediated by progesterone receptor. Our findings might give a new insight into immunomodulatory function of progesterone at fetomaternal interface. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10467408
- Volume :
- 61
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- American Journal of Reproductive Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 36472375
- Full Text :
- https://doi.org/10.1111/j.1600-0897.2008.00684.x