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Post-translational regulation of an Aplysia glutamate transporter during long-term facilitation.
- Source :
- Journal of Neurochemistry; Jan2009, Vol. 108 Issue 1, p176-189, 14p, 9 Color Photographs, 1 Black and White Photograph, 6 Graphs
- Publication Year :
- 2009
-
Abstract
- Regulation of glutamate transporters accompanies plasticity of some glutamatergic synapses. The regulation of glutamate uptake at the Aplysia sensorimotor synapse during long-term facilitation (LTF) was investigated. Previously, increases in levels of ApGT1 ( Aplysia glutamate transporter 1) in synaptic membranes were found to be related to long-term increases in glutamate uptake. In this study, we found that regulation of ApGT1 during LTF appears to occur post-translationally. Serotonin (5-HT) a transmitter that induces LTF did not increase synthesis of ApGT1. A pool of ApGT1 appears to exist in sensory neuron somata, which is transported to the terminals by axonal transport. Blocking the rough endoplasmic reticulum-Golgi-trans-Golgi network (TGN) pathway with Brefeldin A prevented the 5-HT-induced increase of ApGT1 in terminals. Also, 5-HT produced changes in post-translational modifications of ApGT1 as well as changes in the levels of an ApGT1-co-precipitating protein. These results suggest that regulation of trafficking of ApGT1 from the vesicular trafficking system (rough endoplasmic reticulum-Golgi-TGN) in the sensory neuron somata to the terminals by post-translational modifications and protein interactions appears to be the mechanism underlying the increase in ApGT1, and thus, glutamate uptake during memory formation. [ABSTRACT FROM AUTHOR]
- Subjects :
- GLUTAMIC acid
APLYSIA
SEROTONIN
ENDOPLASMIC reticulum
GOLGI apparatus
CELL membranes
Subjects
Details
- Language :
- English
- ISSN :
- 00223042
- Volume :
- 108
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 35553531
- Full Text :
- https://doi.org/10.1111/j.1471-4159.2008.05757.x