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Genome-wide identification of pathogenicity genes in Xanthomonas oryzae pv. oryzae by transposon mutagenesis.
- Source :
- Plant Pathology; Dec2008, Vol. 57 Issue 6, p1136-1145, 10p, 2 Black and White Photographs, 3 Diagrams, 1 Chart
- Publication Year :
- 2008
-
Abstract
- A transposon mutant library was constructed from the bacterial blight pathogen Xanthomonas oryzae pv . oryzae ( Xoo) KACC10331 by Tn5 transposon mutagenesis. The susceptible rice cultivar Milyang 23 was inoculated with a total of 24 540 mutants resistant to kanamycin and 67 avirulent or reduced-pathogenicity mutant strains were selected for study. Southern hybridization verified that 84 mutant strains had single-copy insertions and their single-transposon insertion sites were identified by sequencing analysis combined with thermal asymmetric interlaced (TAIL)-PCR. The single-transposon-tagged sequences of 21 mutant strains belonged to pathogenicity-related genes previously reported in Xanthomonas species, while the other 46 single-transposon-tagged sequences included diverse functional genes encoding, five cell-wall-degrading enzymes, three fimbrial and flagella assembly regulators, five regulatory proteins, 15 metabolic regulators and 18 hypothetical proteins, which were identified as novel pathogenicity genes of Xoo. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00320862
- Volume :
- 57
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Plant Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 35324371
- Full Text :
- https://doi.org/10.1111/j.1365-3059.2008.01884.x