Back to Search
Start Over
Stimulation of dorsal root ganglion neurons activity by pancreatic cancer cell lines
- Source :
- Cell Biology International; Dec2008, Vol. 32 Issue 12, p1530-1535, 6p
- Publication Year :
- 2008
-
Abstract
- Abstract: Stimulation of mice dorsal root ganglion neurons (DRGNs) activity by human pancreatic cancer (PanCa) cell line Mia PaCa-2 and its potential molecule mechanism has been investaged. DRGNs were cultured alone or along with the MIA PaCa-2. The effects of MIA PaCa-2 to DRGNs were determined by neurofilament (NF) immunocytochemical and Nissl staining. ELISA was used to detect the concentration of insulin-like growth factor-1 (IGF-1) in the culture supernatant. Cyton size, neurite outgrowth and neuronal activity in the experimental group were greater than in the control groups. However, the concentration of IGF-1 in the supernatants was not significantly different from those in the blank and non-cultured medium groups. In the presence of MIA PaCa-2 cell line, cyton size, neurite outgrowth and neuronal activity were enhanced, which may provide more routes for the invasion of cancer cells along nerves. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 10656995
- Volume :
- 32
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Cell Biology International
- Publication Type :
- Academic Journal
- Accession number :
- 35291302
- Full Text :
- https://doi.org/10.1016/j.cellbi.2008.08.022