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Is lack of susceptible recipients in the intestinal environment the limiting factor for transduction of Shiga toxin-encoding phages?
- Source :
- Journal of Applied Microbiology; Oct2008, Vol. 105 Issue 4, p1114-1120, 7p, 2 Black and White Photographs, 1 Diagram, 1 Graph
- Publication Year :
- 2008
-
Abstract
- Aim: To determine whether a Shiga toxin 2 (Stx2)-encoding phage from Escherichia coli O157:H7 could be transmitted to commensal E. coli in a ruminant host without adding a specific recipient strain. Methods and Results: Sheep were inoculated with an E. coli O157:H7 strain containing an Stx2-encoding bacteriophage (Φ3538) in which a chloramphenicol-resistant gene, cat, is inserted into stx<subscript>2</subscript>. A total of 149 faecal samples were sampled and analysed for detection and quantification of E. coli O157:H7 and presumptive transductants. Phage Φ3538 (Δ stx<subscript>2</subscript>:: cat) was demonstrated to be transduced to an ovine E. coli O175:H16 at one occasion. Conclusions: The study demonstrates an in vivo transduction in sheep from an E. coli O157:H7 strain to an ovine E. coli O175:H16. A functional Stx2-encoding phage was incorporated into the host’s DNA. Significance and Impact of the Study: This is the first in vivo stx phage transduction study reported in which a recipient strain was not fed to the test animals. We suggest that the access to susceptible hosts is one main limiting factor for transduction to occur in the intestine. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13645072
- Volume :
- 105
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Journal of Applied Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 34319714
- Full Text :
- https://doi.org/10.1111/j.1365-2672.2008.03845.x