Comparison Techniques For HPLC Column Performance.

The article considers the different methods that can be used to compare the performance of liquid chromatography columns and evaluates the advantage of the high pressures and high temperatures some of them can be used at. The main focus is on the kinetic plot method. This method allows the user to transform the more common Van Deemter curve into a curve that describes the ultimate separation speed as a function of the required peak capacity or resolution.