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Is kinase activity essential for biological functions of BRI1?
- Source :
- Cell Research; Apr2008, Vol. 18 Issue 4, p472-478, 7p, 1 Color Photograph, 1 Black and White Photograph, 1 Diagram, 2 Graphs
- Publication Year :
- 2008
-
Abstract
- Brassinosteroids (BRs) are a major group of plant hormones that regulate plant growth and development. BRI1, a protein localized to the plasma membrane, functions as a BR receptor and it has been proposed that its kinase activity has an essential role in BR-regulated plant growth and development. Here we report the isolation and molecular characterization of a new allele of bri1, bri1–301, which shows moderate morphological phenotypes and a reduced response to BRs under normal growth conditions. Sequence analysis identified a two-base alteration from GG to AT, resulting in a conversion of 989G to 989I in the BRI1 kinase domain. An in vitro assay of kinase activity showed that bri1-301 has no detectable autophosphorylation activity or phosphorylation activity towards the BRI1 substrates TTL and BAK1. Furthermore, our results suggest that bri1-301, even with extremely impaired kinase activity, still retains partial function in regulating plant growth and development, which raises the question of whether BRI1 kinase activity is essential for BR-mediated growth and development in higher plants.Cell Research (2008) 18:472–478. doi: 10.1038/cr.2008.36; published online 11 March 2008 [ABSTRACT FROM AUTHOR]
- Subjects :
- BRASSINOSTEROIDS
PLANT hormones
STEROIDS
BRASSINOLIDE
PHOSPHORYLATION
Subjects
Details
- Language :
- English
- ISSN :
- 10010602
- Volume :
- 18
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Cell Research
- Publication Type :
- Academic Journal
- Accession number :
- 31514249
- Full Text :
- https://doi.org/10.1038/cr.2008.36