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Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway.

Authors :
Taketa, Shin
Amano, Satoko
Tsujino, Yasuhiro
Sato, Tomohiko
Saisho, Daisuke
Kakeda, Katsuyuki
Nomura, Mika
Suzuki, Toshisada
Matsumoto, Takashi
Sato, Kazuhiro
Hiroyuki Kanamori
Kawasaki, Shinji
Takeda, Kazuyoshi
Source :
Proceedings of the National Academy of Sciences of the United States of America; 3/11/2008, Vol. 105 Issue 10, p4062-4067, 6p, 4 Graphs
Publication Year :
2008

Abstract

In contrast to other cereals, typical barley cultivars have caryopses with adhering hulls at maturity, known as covered (hulled) barley. However, a few barley cultivars are a free-threshing variant called naked (hulless) barley. The covered/naked caryopsis is controlled by a single locus (nud) on chromosome arm 7HL. On the basis of positional cloning, we concluded that an ethylene response factor (ERF) family transcription factor gene controls the covered/naked caryopsis phenotype. This conclusion was validated by (I) fixation of the 17-kb deletion harboring the ERF gene among all 100 naked cultivars studied; (ii) two x-ray-induced nud alleles with a DNA lesion at a different site, each affecting the putative functional motif; and (iii) gene expression strictly localized to the testa. Available results indicate the monophyletic origin of naked barley. The Nud gene has homology to the Arabidopsis WIN1/SHN1 transcription factor gene, whose deduced function is control of a lipid biosynthesis pathway. Staining with a lipophilic dye (Sudan black B) detected a lipid layer on the pericarp epidermis only in covered barley. We infer that, in covered barley, the contact of the caryopsis surface, overlaid with lipids to the inner side of the hull, generates organ adhesion. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00278424
Volume :
105
Issue :
10
Database :
Complementary Index
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Publication Type :
Academic Journal
Accession number :
31503079
Full Text :
https://doi.org/10.1073/pnas.0711034105