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Prevalence of IgE-Binding to Art v 1, Art v 4 and Amb a 1 in Mugwort-Allergic Patients.
- Source :
- International Archives of Allergy & Immunology; 2008, Vol. 145 Issue 2, p94-101, 8p, 3 Black and White Photographs, 2 Charts
- Publication Year :
- 2008
-
Abstract
- Background: Mugwort (Artemisia vulgaris) represents an important source of weed pollen allergens. The objectives of the present study were (i) to analyze the IgE binding profiles in a group of mugwort-allergic patients, (ii) to identify individual marker allergens crucial for the diagnosis of mugwort allergy and (iii) to identify potential crossreactive allergens present in ragweed (Ambrosia artemisiifolia) pollen extract. Methods: Sera from 100 pediatric mugwort-allergic patients were analyzed for their IgE binding pattern to natural mugwort and ragweed pollen proteins, purified natural and recombinant Art v 1, recombinant Art v 4 and recombinant Amb a 1 using immunoblots and ELISA. Results: 91% of the patients’ sera tested displayed IgE binding to one or more mugwort pollen allergens in ELISA and 88% were positive in immunoblot. Purified natural Art v 1 was recognized by 79%, the recombinant protein by 39% of the patients tested and purified recombinant Art v 4 by 34% of the patients’ sera. 67% of the sera displayed crossreactive IgE to one or more ragweed pollen allergens. Recombinant Amb a 1 was noted in only 14% of the mugwort-allergic sera. Conclusions: Allergen-specific in vitro diagnosis was performed in 100 pediatric mugwort-allergic serum samples. Using two allergens (Art v 1 and Art v 4), 91% of the patients could be identified as mugwort pollen-sensitized patients by IgE in vitro tests. Crossreactivity to ragweed pollen allergens was demonstrated by in vitro experiments, suggesting a new important and potent allergen source expanding across Europe. Copyright © 2007 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Subjects :
- ARTEMISIA vulgaris
ALLERGENS
IMMUNOGLOBULIN E
ANTIGENS
POLLEN
Subjects
Details
- Language :
- English
- ISSN :
- 10182438
- Volume :
- 145
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- International Archives of Allergy & Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 28548116
- Full Text :
- https://doi.org/10.1159/000108134