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Defining an inhibitory domain in the α-subunit of the epithelial sodium channel.
- Source :
- American Journal of Physiology: Renal Physiology; Jan2008, Vol. 294, pF47-F52, 6p, 1 Chart, 2 Graphs
- Publication Year :
- 2008
-
Abstract
- Epithelial sodium channels (ENaC) are processed by proteases as they transit the biosynthetic pathway. We recently observed that furin-dependent processing of the α-subunit of ENaC at two sites within its extracellular domain is required for channel activation due to release of a 26-residue inhibitory domain. While channels with α-subunits lacking the furin sites are not cleaved and have very low activity, channels lacking the furin consensus sites as well as the tract between these sites (αD206 -R23 1) are active. We analyzed channels with a series of deletions in the tract αD206-R231 and lacking the α-subunit furin consensus sites in Xenopus laevis oocytes. We found an eight-residue tract that, when deleted, restored channel activity to the level found in oocytes expressing wild-type ENaC. A synthetic peptide, LPHPLQRL, representing the tract αL211-L218, inhibited wild-type ENaC expressed in oocytes with an IC<subscript>50</subscript> of 0.9 μ.M, and inhibited channels expressed in collecting duct cells and human primary airway epithelial cells with an IC<subscript>50</subscript>s of between ∼50 and 100 μM. Analyses of peptides with deletions within this inhibitory tract indicate that eight residues is the minimal backbone length that is required for ENaC inhibition. Analyses of 8-mer peptides with conserved and nonconserved substitutions suggest that L¹, P², H³, P<superscript>4</superscript>, and L<superscript>8</superscript> are required for inhibitory activity. Our findings suggest that this eight-residue tract is a key conserved inhibitory domain that provides epithelial cells with a reserve of inactive channels that can be activated as required by proteases. [ABSTRACT FROM AUTHOR]
- Subjects :
- SODIUM channels
ION channels
PEPTIDES
EPITHELIAL cells
PROTEOLYTIC enzymes
EPITHELIUM
Subjects
Details
- Language :
- English
- ISSN :
- 1931857X
- Volume :
- 294
- Database :
- Complementary Index
- Journal :
- American Journal of Physiology: Renal Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 28529205
- Full Text :
- https://doi.org/10.1152/ajprenal.00399.2007