EFFECT OF RADIX SCUTELLARIAE ON THE PROLIFERATION OF THE BASAL-LIKE BREAST CANCER LINE.

Objective: As the representative of Chinese herb for invigorating qi, Radix scutellariae is frequently used to treat breast cancer in clinical. This study investigated the effect of Radix scutellariae injection (RSI) on the basal-like breast cancer cell line MDA-MB-468 and murine bone marrow stromal stem cells (mMSCs) to validate the Radix scutellariae can anticancer and does not damage the normal cells. Method: Different concentrations of RSIs were used to treat MDA-MB-468 cells and primary cultured mMSCs. The morphology of cells was observed by phase-contrast invert microscopy (PCIM) and transmission electron microscopy (TEM). LDH levels were inspected in the supernatants. WST-8 assay was used to determine the dose-response relationship and time-response relationship. The dose-response relationship was determined to be average value of MDA-MB-468 that was treated by the different concentrations of RSIs for 2 days, and the time-response relationship was determined from the first day through the seventh day. The percentages of the cell-cycle phases and the apoptosis were detected by flow cytometry (FCM). Results: (a) We found that the junctures of the cells were tight, but the cell membranes had been broken when MDA-MB-468 cells were treated by the different concentrations of RSI. With TEM, we can observe a number of death cells of 1 g/mL concentration RSI group. The active cells had shorter and less microfilament, but their nucleoli and organelle unchanged. Those morphologic changes are not obvious in 10^-1 g/mL concentration RSI group. LDH lever of the HRSI group was 42.50 [138} 3.27 and that of the LRSI group was 54.50 ± 3.08. They compared with that of the control group, 55.17 ± 7.25, p > .05. (b) With WST-8 assay, A value of MDA-MB-468 treated by LRSI for 2 days was 0.06 ± 0.01 and that of the HRSI group was 0.05 ± 0.01, compared with the control group 0.08 ± 0.01, p < .05 and p < .005, respectively. But A value of 10^-2 g/mL concentration RSI group was 0.1 ± 0.02, p < .05. (c) We also determined average value of MDA-MB-468 with RSIs from the first day through the seventh day. That of HRSI group were d1 (0.20 ± 0.02), d2 (0.28 ± 0.03), d3 (0.21 ± 0.02), d4 (0.16 ± 0.02), d5 (0.14 ± 0.02), d6 (0.08 ± 0.01), d7 (0.07 ± 0.01), in turn, and that of the control group was dl (0.29 ± 0.01), d2 (0.65 ± 0.05), d3 (0.57 ± 0.07), d4 (0.69 ± 0.04), d5 (0.68 ± 0.04), d6 (0.63 ± 0.04), d7 (0.85 ± 0.02), p < .001. A value of LRSI group only at the fourth day was 0.57 ± 0.02, lower than that of the control group, p < .05. At the first, sixth, and seventh day, A value was 0.38 -± 0.03, 0.84 ± 0.04, and 0.93 ± 0.02, in turn, higher than that of the control group, p < .001. At the second, third, and fifth day, average value had insignificant differentia compared with the control group, p > .05. (d) With the above method, A value of mMSCs treated by HRSI for 2 days was 0.06 ± 0.01, compared with the control group 0.11 ± 0.0l, p < .005. But that of the LRSI group was 0.18 ± 0.02, higher than the control group, p < .01. (e) Detected by FCM, the percentage of apoptosis of the HRSI group was 17.24%, higher than that of the control group, 2.3%. The percent of G2-M phase was 59.86% versus 25.24%. Therefore, that of the LRSI group was 1.92% and 29.46%, respectively. Conclusions: 1 g/mL and 10^-1 g/mL concentration RSIs both inhibited the proliferation of MDA-MB-468 cells. And the inhibiting effect of RSI strengthened with the increase in concentration. But 10^-2 g/ml RS1 promoted the cells' proliferation.… [ABSTRACT FROM AUTHOR]