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Reorganization of prion protein membrane environment during low potassium-induced apoptosis in primary rat cerebellar neurons.
- Source :
- Journal of Neurochemistry; Dec2007, Vol. 103 Issue 5, p1954-1967, 14p, 4 Black and White Photographs, 1 Diagram, 6 Graphs
- Publication Year :
- 2007
-
Abstract
- We studied the changes occurring in the membrane environment of prion protein (PrP) during apoptosis induced by low potassium in primary rat cerebellar neurons. Ceramide levels increased during apoptosis-inducing treatment, being doubled with respect to time-matched controls after 24 h. Sphingomyelin levels were parallely decreased, while cholesterol and ganglioside contents were not affected. Changes in ceramide and sphingomyelin composition were exclusively restricted to a detergent-resistant membrane fraction. The pro-apoptotic treatment was accompanied by the down-regulation of PrP and of the non-receptor kinase Fyn. The levels of PrP and Fyn were correspondingly reduced in the detergent-resistant membrane fraction. In control cells, the membrane microenvironment separated by immunoprecipitation with anti-PrP antibody contained 80% of the detergent-resistant PrP and 35% and 38% of the sphingolipids and cholesterol respectively. Upon low potassium treatment, 20% of the PrP originally present in the detergent-resistant fraction was immunoprecipitated, together with 19% of sphingolipids and 22% of cholesterol. Thus, PrP in the immunoprecipitate from apoptotic cells was ninefold less than in control ones, while sphingolipids and cholesterol were about 50% with respect to controls cells. The molar ratio between cholesterol, sphingomyelin and ceramide was 15 : 6 : 1 in the PrP-rich environment from control neurons, and 6 : 2 : 1 in that from apoptotic cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- APOPTOSIS
POTASSIUM
CERAMIDES
SPHINGOLIPIDS
NEURONS
CELL death
Subjects
Details
- Language :
- English
- ISSN :
- 00223042
- Volume :
- 103
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Journal of Neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 27476594
- Full Text :
- https://doi.org/10.1111/j.1471-4159.2007.04890.x