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Development of automated amperometric detection of antibodies against Bacillus anthracis protective antigen.
- Source :
- Analytical & Bioanalytical Chemistry; Sep2007, Vol. 389 Issue 2, p507-515, 9p, 2 Charts, 4 Graphs
- Publication Year :
- 2007
-
Abstract
- Picogram levels of antibodies against the protective antigen (PA) of Bacillus anthracis were detected in an automated electrochemical sandwich-type enzyme-linked immunosorbant assay. The antibodies were captured and detected using an 8 × 3 array of 50-μm-diameter cavities. The reagent and sample volumes were as low as 200 nL in a less than 25-min assay from capture to signal generation. The electrochemical detection of the antibodies was demonstrated at 0.05–10 μg/mL containing only 10–5,000 pg antibodies. The limit of detection is 10 fg for a 200-nL sample. Detection of anti-PA immunoglobulin G performed in spiked normal human serum and fresh whole human blood did not show a significant difference from detection in a buffer. The initial automation of the assay involved the use of a digital syringe pump for the delivery of reagents to the capture surface. [ABSTRACT FROM AUTHOR]
- Subjects :
- CONDUCTOMETRIC analysis
BACILLUS anthracis
ANTIGENS
MICROELECTRODES
IMMUNOASSAY
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 389
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 26340094
- Full Text :
- https://doi.org/10.1007/s00216-007-1448-z