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Immunohistochemical identification of type I and type III collagen and chondroitin sulphate in human pre-dentine: a correlative FEI-SEM/TEM study.
- Source :
- International Endodontic Journal; Sep2007, Vol. 40 Issue 9, p669-678, 10p, 3 Black and White Photographs
- Publication Year :
- 2007
-
Abstract
- Aim To identify type I- (I-CF) and type III-collagen fibrils (III-CF) and chondroitin 4/6 sulphate (CS) within human pre-dentine by means of a correlative analysis under field emission in-lens-scanning electron microscopy (FEI-SEM) and transmission electron microscopy (TEM). Methodology Human-extracted teeth were obtained and submitted to either a pre-embedding or a post-embedding immunolabelling procedure using monoclonal primary antibodies anti-I-CF, anti-III-CF and anti-CS. Gold-conjugated secondary antibodies were coupled to primary antibodies to visualize labelling under the electron beam. Correlative labelling patterns were obtained for I-CF and CS under both FEI-SEM and TEM. Results Field emission in lens-SEM analysis revealed an intricate three-dimensional network of I-CF and CS clarifying the intimate relationship between the two main components of the pre-dentine organic matrix. TEM analysis revealed odontoblasts exhibiting intracellular labelling for CS, which became more intense and diffuse over the pre-dentine organic matrix. The same diffuse immunoreaction was revealed for I-CF, whereas a weak immunolocalization of III-CF was found scattered throughout the pre-dentine layer and over the collagen fibrils. Conclusions Both the pre- and post-embedding immunohistochemical approaches have led to the visualization of CF- and CS-labelling distribution within the pre-dentine layer, adding further knowledge on the elucidation of collagen–proteoglycans interaction in the organic matrix of human dental roots. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01432885
- Volume :
- 40
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- International Endodontic Journal
- Publication Type :
- Academic Journal
- Accession number :
- 26149142
- Full Text :
- https://doi.org/10.1111/j.1365-2591.2007.01263.x