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Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen.

Authors :
Tatsuya Munaka
Hirohisa Abe
Masaki Kanai
Takashi Sakamoto
Hiroaki Nakanishi
Shuichi Shoji
Shinya Kimura
Taira Maekawa
Akira Murakami
Source :
Analyst; May2007, Vol. 132 Issue 6, p512-514, 3p
Publication Year :
2007

Abstract

This paper describes the cultivation of primary cells in a microchamber and the real-time monitoring of small amounts of antibody secretion after the introduction of a minute amount of stimulus by a microinjector using time-resolved fluorescence anisotropy analysis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00032654
Volume :
132
Issue :
6
Database :
Complementary Index
Journal :
Analyst
Publication Type :
Academic Journal
Accession number :
25208714
Full Text :
https://doi.org/10.1039/b701049d