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Nanoscale Fabrication of P. aeruginosa Azurin on Self-Assembled Monolayer.
- Source :
- Molecular Crystals & Liquid Crystals; 2007, Vol. 463 Issue 1, p281-289, 9p, 1 Diagram, 1 Graph
- Publication Year :
- 2007
-
Abstract
- Pseudomonas aeruginosa Azurin is a metalloprotein of 14 kDa with copper ion in its active site. Protein film formation onto the substrate was performed by the self-assembly technique. In order to make molecule assembled layer onto the gold substrate, Azurin was cross-linked with N-succinimidyl-3-(2-pyridyldithio)-propionate (SPDP). SPDP have both a -thiol group to assembly with gold substrate and a linking part to assembly with protein. We detected optimized condition to make a protein film using Surface Plasmon Resonance (SPR). The surface structure of adsorbed Azurin film onto the gold surface was analyzed by scanning tunneling microscope (STM). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15421406
- Volume :
- 463
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Molecular Crystals & Liquid Crystals
- Publication Type :
- Academic Journal
- Accession number :
- 24333769
- Full Text :
- https://doi.org/10.1080/15421400601028369