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Nanoscale Fabrication of P. aeruginosa Azurin on Self-Assembled Monolayer.

Authors :
Jeong-Woo Choi
Jin Seok Kim
Young Hark Jang
Bum Hwan Lee
Young Jun Kim
Source :
Molecular Crystals & Liquid Crystals; 2007, Vol. 463 Issue 1, p281-289, 9p, 1 Diagram, 1 Graph
Publication Year :
2007

Abstract

Pseudomonas aeruginosa Azurin is a metalloprotein of 14 kDa with copper ion in its active site. Protein film formation onto the substrate was performed by the self-assembly technique. In order to make molecule assembled layer onto the gold substrate, Azurin was cross-linked with N-succinimidyl-3-(2-pyridyldithio)-propionate (SPDP). SPDP have both a -thiol group to assembly with gold substrate and a linking part to assembly with protein. We detected optimized condition to make a protein film using Surface Plasmon Resonance (SPR). The surface structure of adsorbed Azurin film onto the gold surface was analyzed by scanning tunneling microscope (STM). [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
15421406
Volume :
463
Issue :
1
Database :
Complementary Index
Journal :
Molecular Crystals & Liquid Crystals
Publication Type :
Academic Journal
Accession number :
24333769
Full Text :
https://doi.org/10.1080/15421400601028369