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Regulation of galectin-1 expression by transforming growth factor β1 in metastatic mammary adenocarcinoma cells: implications for tumor-immune escape.

Authors :
Daroqui, Cecilia M.
Ilarregui, Juan M.
Rubinstein, Natalia
Salatino, Mariana
Toscano, Marta A.
Vazquez, Paula
Bakin, Andrei
Puricelli, Lydia
Bal de Kier Joffé, Elisa
Rabinovich, Gabriel A.
Source :
Cancer Immunology, Immunotherapy; Apr2007, Vol. 56 Issue 4, p491-499, 9p, 1 Diagram, 1 Chart, 5 Graphs
Publication Year :
2007

Abstract

Tumors escape from immune surveillance by producing immunosuppressive cytokines and proapototic factors, including TGF-β and galectin-1 (Gal-1). Since immunosuppressive mechanisms might act in concert to confer tumor-immune privilege, we investigated the potential cross talk between TGF-β and Gal-1 in highly metastatic mammary adenocarcinoma (LM3) cells. While Gal-1 treatment was not capable of regulating TGF-β synthesis, a pronounced and dose-dependent increase in Gal-1 expression was observed when tumor cells were treated with TGF-β<subscript>1. </subscript>This effect was also observed in the murine lung adenocarcinoma LP07 and in the human breast adenocarcinoma MCF-7 cell lines. TGF-β1-mediated upregulation of Gal-1 expression was specifically mediated by TβRI and TβRII, since it was abrogated when LM3 cells were infected with retroviral vectors expressing the dominant negative forms of these receptors. In addition, gal-1 gene sequence analysis revealed the presence of three putative binding sites for Smad4 and Smad3 transcription factors, consistent with the ability of TGF-β<subscript>1</subscript> to trigger a Smad-dependent signaling pathway in these cells. Thus, TGF-β<subscript>1</subscript> may trigger a Smad-dependent pathway to control Gal-1 expression, suggesting that distinct mechanisms might cooperate in tilting the balance toward an immunosuppressive environment at the tumor site. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03407004
Volume :
56
Issue :
4
Database :
Complementary Index
Journal :
Cancer Immunology, Immunotherapy
Publication Type :
Academic Journal
Accession number :
23857707
Full Text :
https://doi.org/10.1007/s00262-006-0208-9