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Induction of CD16+ CD56bright NK Cells with Antitumour Cytotoxicity not only from CD16− CD56bright NK Cells but also from CD16− CD56dim NK Cells.
- Source :
- Scandinavian Journal of Immunology; Feb2007, Vol. 65 Issue 2, p126-138, 13p, 3 Diagrams, 2 Graphs
- Publication Year :
- 2007
-
Abstract
- The aim of this study was to examine the effect of cytokines on different subsets of NK cells, while especially focusing on CD16<superscript>−</superscript> CD56<superscript>dim</superscript> cells and CD16<superscript>−</superscript> CD56<superscript>bright</superscript> cells. When human peripheral blood mononuclear cells (PBMC) were cultured with a combination of IL-2, IL-12 and IL-15 for several days, a minor population of CD56<superscript>bright</superscript> NK cells expanded up to 15%, and also showed potent cytotoxicities against various cancer cells. Sorting experiments revealed that unconventional CD16<superscript>−</superscript> CD56<superscript>+</superscript> NK cells (CD16<superscript>−</superscript> CD56<superscript>dim</superscript> NK cells and CD16<superscript>−</superscript> CD56<superscript>bright</superscript> NK cells, both of which are less than 1% in PBMC) much more vigorously proliferated after cytokine stimulation, whereas predominant CD16<superscript>+</superscript> CD56<superscript>dim</superscript> NK cells proliferated poorly. In addition, many of the resting CD16<superscript>−</superscript> CD56<superscript>bright</superscript> NK cells developed into CD16<superscript>+</superscript> CD56<superscript>bright</superscript> NK cells, and CD16<superscript>−</superscript> CD56<superscript>dim</superscript> NK cells developed into CD16<superscript>−</superscript> CD56<superscript>bright</superscript> NK cells and also further into CD16<superscript>+</superscript> CD56<superscript>bright</superscript> NK cells by the cytokines. CSFE label experiments further substantiated the proliferation capacity of each subset and the developmental process of CD16<superscript>+</superscript> CD56<superscript>bright</superscript> NK cells. Both CD16<superscript>−</superscript> CD56<superscript>dim</superscript> NK cells and CD16<superscript>−</superscript> CD56<superscript>bright</superscript> NK cells produced large amounts of IFN- γ and Fas-ligands. The CD16<superscript>+</superscript> CD56<superscript>bright</superscript> NK cells showed strong cytotoxicities against not only MHC class I (−) but also MHC class I (+) tumours regardless of their expression of CD94/NKG2A presumably because they expressed NKG2D as well as natural cytotoxicity receptors. The proliferation of CD16<superscript>+</superscript> CD56<superscript>bright</superscript> NK cells was also induced when PBMC were stimulated with penicillin-treated Streptococcus pyogenes, thus suggesting their role in tumour immunity and bacterial infections. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03009475
- Volume :
- 65
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Scandinavian Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 23791737
- Full Text :
- https://doi.org/10.1111/j.1365-3083.2006.01883.x