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Mutations in Plasmodium falciparum Chloroquine Resistance Transporter and Multidrug Resistance Genes, and Treatment Outcomes in Ghanaian Children with Uncomplicated Malaria.
- Source :
- Journal of Tropical Pediatrics; Feb2007, Vol. 53 Issue 1, p27-31, 5p
- Publication Year :
- 2007
-
Abstract
- The association between the clinical outcome of chloroquine treatment and mutations in the putative Plasmodium falciparum chloroquine resistance transporter (Pfcrt) gene at codon 76 and multidrug resistance gene 1 (Pf mdr1) at codon 86 were investigated among 406 children with uncomplicated malaria presenting at five sentinel health centres in Ghana. Presence of mutations in isolates taken at pre-treatment and on day of recurrence of parasites was detected using PCR followed by RFLP techniques. The prevalence of Pfcrt T76 mutants was 80% at Hohoe, 46% at Navrongo, 98% at Tarkwa, 61% at Sunyani and 46% at Yendi. The prevalence of the mutant Pfmdr1 at Hohoe, Navrongo, Tarkwa, Sunyani and Yendi were 78, 58, 95, 53 and 42%, respectively. Significant association between the Pfcrt mutation and treatment outcome was observed at Hohoe and Sunyani (p < 0.05), but not at Navrongo, Tarkwa or Yendi (p > 0.05). Similarly, a statistical significant association between Pfmdr1 86 and treatment failures was observed at Hohoe and Sunyani (p < 0.05) but not at the other three sites. A positive correlation was found between mutant Pfcrt prevalence only and treatment failures with a Spearman's ρ-value of 0.872 and a p-value = 0.027. All parasite isolates from samples taken at recrudescence from patients with chloroquine treatment failures were found to have both Pfcrt and Pfmdr mutations. [ABSTRACT FROM AUTHOR]
- Subjects :
- MALARIA treatment
CHLOROQUINE
MULTIDRUG resistance
Subjects
Details
- Language :
- English
- ISSN :
- 01426338
- Volume :
- 53
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Tropical Pediatrics
- Publication Type :
- Academic Journal
- Accession number :
- 23787281