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Antibodies from dogs with canine visceral leishmaniasis recognise two proteins from the saliva of Lutzomyia longipalpis.

Authors :
Diana Bahia
Nelder Gontijo
Ileana León
Jonas Perales
Marcos Pereira
Guilherme Oliveira
Rodrigo Corrêa-Oliveira
Alexandre Reis
Source :
Parasitology Research; Feb2007, Vol. 100 Issue 3, p449-454, 6p
Publication Year :
2007

Abstract

Abstract??The saliva of the sand flyLutzomyia longipalpis,a major vector ofLeishmania, exhibits pharmacological and immunomodulatory activities that may facilitate entry and establishment of parasites into the vertebrate host. Salivary gland components of the sand fly are, therefore, potential candidates in the development of a vaccine against human leishmaniasis. With the objective of identifying sand fly saliva proteins that could be used to immunise animals against canine visceral leishmaniasis, we have evaluated anti-saliva antibody reactivity using serum samples collected from dogs naturally infected withLeishmania chagasi.Two proteins with molecular weights of 28.6 and 47.3?kDa were recognised by dog antibodies in Western blot assays. Protein bands were excised from an SDS-PAGE gel and the sequences determined by mass spectrometry. The proteins were identified as LuLo-D7 and Lulo YELLOW, respectively. The significance of these findings in the context of the development of multi-component vaccination experiments is discussed. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09320113
Volume :
100
Issue :
3
Database :
Complementary Index
Journal :
Parasitology Research
Publication Type :
Academic Journal
Accession number :
23650191