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Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome.
- Source :
- Molecular Microbiology; Jul2006, Vol. 61 Issue 2, p394-406, 13p, 1 Color Photograph, 3 Diagrams, 1 Chart, 3 Graphs
- Publication Year :
- 2006
-
Abstract
- In Escherichia coli the initial step in the processing or decay of many messenger and structural RNAs is mediated by the endonuclease RNase E, which forms the core of a large RNA-catalysis machine termed the degradosome. Previous experiments have identified a protein that globally modulates RNA abundance by binding to RNase E and regulating its endonucleolytic activity. Here we report the discovery of RraB, which interacts with a different site on RNase E and interferes with cleavage of a different set of transcripts. We show that expression of RraA or RraB in vivo is accompanied by dramatic, distinct, and inhibitor-specific changes in degradosome composition – and that these are in turn associated with alterations in RNA decay and global transcript abundance profiles that are dissimilar to the profile observed during simple RNase E deficiency. Our results reveal the existence of endonuclease binding proteins that modulate the remodelling of degradosome composition in bacteria and argue that such degradosome remodelling is a mechanism for the differential regulation of RNA cleavages in E. coli. [ABSTRACT FROM AUTHOR]
- Subjects :
- ESCHERICHIA coli
FUNGUS-bacterium relationships
BACTERIA
RNA
NUCLEIC acids
Subjects
Details
- Language :
- English
- ISSN :
- 0950382X
- Volume :
- 61
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Molecular Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 21362281
- Full Text :
- https://doi.org/10.1111/j.1365-2958.2006.05246.x