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Transglutaminase differentially regulates growth signalling in rat perivenous and periportal hepatocytes.
- Source :
- Cell Proliferation; Jun2006, Vol. 39 Issue 3, p183-193, 11p, 1 Chart, 5 Graphs
- Publication Year :
- 2006
-
Abstract
- The influence of transglutaminase 2 (TG2) activity on the proliferative effect of epidermal growth factor (EGF) and on EGF receptor affinity in periportal hepatocytes (PPH) and perivenous hepatocytes (PVH) has been investigated using a primary culture system. PPH and PVH subpopulations have been isolated using the digitonin/collagenase perfusion technique. DNA synthesis was assessed by [<superscript>3</superscript>H] thymidine incorporation into hepatocytes. The assay for binding of [<superscript>125</superscript>I] EGF to cultured hepatocytes was analysed by Scatchard plot analysis. Pretreatment with the TG2 inhibitor monodansylcadaverine (MDC) greatly increased EGF-induced DNA synthesis in both PPH and PVH. Furthermore, [<superscript>125</superscript>I] EGF binding studies in PVH treated with MDC indicated that high-affinity EGF receptor expression was markedly up-regulated, whereas in PPH, there was no significant effect. Treatment with retinoic acid (RA), an inducer of TG2 expression, significantly decreased EGF-induced DNA synthesis in both PPH and PVH. Binding studies in the presence of RA revealed that the high-affinity EGF receptor was down-regulated and completely absent in both PPH and PVH. These results suggest that TG2 was involved in the differential growth capacities of PPH and PVH through down-regulation of high-affinity EGF receptors. [ABSTRACT FROM AUTHOR]
- Subjects :
- TRANSGLUTAMINASES
EPIDERMAL growth factor
LIVER cells
THYMIDINE
TRETINOIN
Subjects
Details
- Language :
- English
- ISSN :
- 09607722
- Volume :
- 39
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Cell Proliferation
- Publication Type :
- Academic Journal
- Accession number :
- 20620725
- Full Text :
- https://doi.org/10.1111/j.1365-2184.2006.00379.x