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Expression, purification, crystallization and preliminary X-ray diffraction of a novel Nitrosomonas europaea cytochrome, cytochrome P460.
Expression, purification, crystallization and preliminary X-ray diffraction of a novel Nitrosomonas europaea cytochrome, cytochrome P460.
- Source :
- Acta Crystallographica: Section F (Wiley-Blackwell); Apr2006, Vol. 62 Issue 4, p395-398, 4p, 1 Color Photograph, 1 Black and White Photograph, 1 Diagram, 1 Chart
- Publication Year :
- 2006
-
Abstract
- Cytochrome P460 from Nitrosomonas europaea, a novel mono-heme protein containing an unusual cross-link between a conserved lysine and the porphyrin ring, has been recombinantly expressed and purified from Escherichia coli. The protein crystallizes readily and diffraction to 1.7 Å has been obtained in-house. The crystals belong to the trigonal space group P3<subscript>1/2</subscript>21, with unit-cell parameters a = b = 53.3, c = 127.1 Å, and contain one monomer in the asymmetric unit. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17443091
- Volume :
- 62
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Acta Crystallographica: Section F (Wiley-Blackwell)
- Publication Type :
- Academic Journal
- Accession number :
- 20386443
- Full Text :
- https://doi.org/10.1107/S1744309106008785