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Codetection of Measles Virus and Tumor Necrosis Factor-Alpha mRNA in Otosclerotic Stapes Footplates.
- Source :
- Laryngoscope; Jul2005, Vol. 115 Issue 7, p1291-1297, 7p
- Publication Year :
- 2005
-
Abstract
- HYPOTHESIS:: Otosclerosis is a disease of unknown etiology causing conductive or sensorineural hearing loss. Persistent measles virus infection of the otic capsule is considered to be one of the etiologic factors in otosclerosis.BACKGROUND:: Determinants of measles virus infection and reactive inflammation were studied in otosclerosis. The presence of measles virus was shown in otosclerotic patients by reverse-transcription polymerase chain reaction (RT-PCR) amplification of the viral RNA. No report is available, however, on the types and features of paracrine cytokines in otosclerosis.METHODS:: Nucleic acid was extracted from stapes footplate samples of clinically otosclerotic patients. Measles virus nucleoprotein RNA was amplified by seminested RT-PCR. Tumor necrosis factor (TNF)-alpha mRNA expression was detected by RT-PCR in otosclerotic bone and was correlated with preoperative audiologic findings and measles virus positivity.RESULTS:: Among 154 clinically otosclerotic patients, 99 stapes footplate specimens contained measles virus RNA. TNF-alpha mRNA was detectable in 88 virus-positive and in 6 virus-negative stapes footplates. There was no detectable TNF-alpha mRNA expression in virus negative cases.CONCLUSION:: The etiologic role of measles virus in the pathogenesis of otosclerosis should be considered. Detection of TNF-alpha mRNA demonstrates activated osteoclast functions and inflammatory pathways in otosclerotic stapes footplates associated with measles virus presence. Virus-associated and virus-negative pathomechanisms of otosclerosis should be distinguished. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0023852X
- Volume :
- 115
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Laryngoscope
- Publication Type :
- Academic Journal
- Accession number :
- 20130063