Thin layer chromatographic resolution of some 2-arylpropionic acid enantiomers using L-(−)-serine, L-(−)-threonine and a mixture of L-(−)-serine and L-(−)-threonine-impregnated silica gel as stationary phases.

Impregnated silica TLC plates with L-(−)-serine and L-(−)-threonine and a mixture of L-(−)-serine and L-(−)-threonine (1:1) as chiral selectors were prepared to use as chiral stationary phases (CSPs) in thin layer chromatography. The resolution of the enantiomers of 2-arylpropionic drugs, including ibuprofen, ibuproxam, ketoprofen, pranoprofen, benoxaprofen, flurbiprofen and tiaprofenic acid was investigated on these CSPs. A mobile phase system of acetonitrile–methanol–water (16:4:0.5, v/v/v) was used. The spots were detected with iodine vapours and the detection limits were found to range between 0.25 and 0.5 µg/mL for all racemic compounds investigated. The effect of temperature, pH and concentration of the impregnating chiral selectors on resolution has been studied. Copyright © 2003 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]