Bovine PrPC directly interacts with αB-crystalline

Abstract: We used a bovine brain cDNA library to perform a yeast two-hybrid assay with bovine mature PrP^C as bait. The screening result showed that αB-crystalline interacted with PrP^C. The interaction was further evaluated both in vivo and in vitro with different methods, such as immunofluorescent colocalization, native polyacrylamide-gel electrophoresis, and IAsys biosensor assays. The results suggested that αB-crystalline may have the ability to refold denatured prion proteins, and provided first evidence that αB-crystalline is directly associated with prion protein. [Copyright &y& Elsevier]