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Recognition of an extensive range of IgE-reactive proteins in cod extract.
- Source :
- Allergy; Jan1998, Vol. 53 Issue 1, p42-50, 9p, 2 Diagrams, 2 Charts, 4 Graphs
- Publication Year :
- 1998
-
Abstract
- Allergy to fish is one of the most common food allergies. Gad c 1 is the only fish allergen which has been purified and characterized. Other allergens have been detected by Western blot in cod extracts. We have now improved the Western-blot procedure in order to characterize fish IgE-reactive proteins from extracts prepared under different conditions: pre-rigor mortis and post-rigor mortis. EDTA addition or not. and DEAE ion-exchange chromatography. Several IgE-reactive protein bands have been identified over a wide molecular-weight range. In particular, the 104- and 130-kDa IgE-reactive protein bands were detected. These new bands may correspond to aggregates, as EDTA increased the relative amount of the 60-. 67-. 104-, and 130-kDa IgE-reactive protein bands in Western blot. All these bands were also detected by an antiparvalbumin monoclonal antibody, specific to the first calcium-binding site. The longer period of storage increased the relative amounts of the 41-, 80-, 104-. and 130-kDa IgE-reactive protein bands. The 18-kDa band was detected only in fish stored for several days. In conclusion, we have described IgE-reactive protein bands over a wide molecular-weight range (12-130 kDa) in Western blot of cod extract, and shown that EDTA and storage conditions may influence the relative distribution of IgE-reactive protein bands. [ABSTRACT FROM AUTHOR]
- Subjects :
- FOOD allergy
CODFISH
IMMUNOGLOBULIN E
ALLERGENS
FISHES
Subjects
Details
- Language :
- English
- ISSN :
- 01054538
- Volume :
- 53
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 18596057
- Full Text :
- https://doi.org/10.1111/j.1398-9995.1998.tb03772.x