Patent Issued for Multiplex amplification detection assay II (USPTO 12173362).

A patent was issued for a multiplex amplification detection assay by Exact Sciences Corporation, developed by inventors from various locations in the United States. The method described in the patent focuses on analyzing samples for multiple target nucleic acids, particularly in low-target samples, using a combination of primer pairs and PCR-flap assays. This innovative approach allows for real-time detection without the need for whole-genome pre-amplification or nested primers, enhancing the accuracy of detecting low copy number targets in complex samples. [Extracted from the article]