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Zn(II) coordination influences the secondary structure, but not antimicrobial activity of the N-terminal histatin 3 hydrolysis product.
- Source :
- Dalton Transactions: An International Journal of Inorganic Chemistry; 12/28/2024, Vol. 53 Issue 48, p19202-19213, 12p
- Publication Year :
- 2024
-
Abstract
- The relationship between the coordination chemistry and antimicrobial activity of Zn(II) and Cu(II)-bound histatins, salivary antimicrobial peptides, remains enigmatic. We focus on metal complexes of histatin 3 and its two products of hydrolysis: histatin 4 and its N-terminal fragment (histatin 3–4). The thermodynamic stability of these complexes is quite expected – the binding of Cu(II) via the ATCUN motif results in the formation of very stable complexes. In histatin-Zn(II) complexes, the {2N<subscript>im</subscript>} type of coordination dominates, with polymorphic binding sites observed for histatin 3–4 and 5–8, resulting in their low thermodynamic stability compared to the complexes of histatin 3, 4, 5 and 8 with Zn(II), in which we observe a {2N<subscript>im</subscript>, O<superscript>−</superscript>} type of coordination. Histatin 3, 3–4 and 4 have greater activity against Gram-positive bacteria than against Gram-negative ones, and Cu(II) or Zn(II) binding can, in some cases, moderately increase the antimicrobial activity of the native histatin 3 and 4, but not the remaining 3–4 fragment. The most probable reason for the metal-enhanced antimicrobial activity is, in this case, a local change of charge, while the chemically fascinating metal binding induced structural changes do not result in a change of biological activity. Neither histatin 3–4, the N-terminal fragment of histatin 3, which remains in solution after cleavage, nor its metal complexes have any antimicrobial activity, but histatin 3–4 presents intriguing Zn(II)-induced structural behavior, changing its secondary structure, with a tendency to form an α-helix. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14779226
- Volume :
- 53
- Issue :
- 48
- Database :
- Complementary Index
- Journal :
- Dalton Transactions: An International Journal of Inorganic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 181524805
- Full Text :
- https://doi.org/10.1039/d4dt02274b