Anti-Cancer Efficacy of Niosomal Encapsulated Withania somnifera on Breast Cancer Cells: An in Vitro Study.

Molecular analysis of breast cancer cells by niosomal encapsulated Withania somnifera (WS) extract is the focus of this investigation. Characterization of ASH-loaded niosomes produced by a thin film technique showed that they have an appropriate zeta potential, small particle size, and low polydispersity index. The concentration-dependent suppression of cell proliferation was seen in MCF-7 breast cancer cells treated with ASH-NIO, with an IC50 of 24.13 µM, and olaparib at values of 10.45 µM. Molecular investigation showed that ASH-NIO treated cells had lower levels of B-cell lymphoma 2 (Bcl2) mRNA expression compared to untreated cells, and upregulated levels of Tumor protein 53 (P53), Bcl-2-associated X protein (Bax), caspase-9, and caspase-3. These results provide more evidence that ASH-NIO has potential anti-cancer effects and may be useful as an adjunctive treatment for breast cancer. [ABSTRACT FROM AUTHOR]