Back to Search Start Over

Evaluation of various polysaccharide‐based stationary phases for enantioseparation of chloro‐containing derivatives in normal phase liquid chromatography.

Evaluation of various polysaccharide‐based stationary phases for enantioseparation of chloro‐containing derivatives in normal phase liquid chromatography.

Authors :
Ghinet, Alina
Furman, Christophe
Zubaş, Andreea
Apostol, Georgiana
Nica, Adrian Sorin
Lipka, Emmanuelle
Source :
Biomedical Chromatography; Dec2024, Vol. 38 Issue 12, p1-15, 15p
Publication Year :
2024

Abstract

Six polysaccharide‐based chiral stationary phases were screened to separate the enantiomers of six chloro‐containing derivatives and one derivative bearing electron donating mesomeric substituents, chosen for comparison. These compounds are expected to be P2X7 receptor antagonists with potential anti‐inflammatory activity. The study was carried out with four different mobile phases composed of n‐heptane and ethanol or isopropanol. Thus, a total of 168 experiments were implemented to find the best conditions aimed at scaling‐up the separation of these anti‐inflammatory compounds. Chiralpak AD‐H separated half of them, i.e., 1, 2, and 6; Chiralpak AS separated also three out of the six compounds, i.e., 1, 2, and 3; Lux Cellulose‐5 separated 2, 4, and 6; Lux Cellulose‐2 separated 1, 2, and 4; Chiralcel OD‐H separated compounds 2 and 5; and finally Chiralcel OJ separated only 3, thus having the lowest rate of success. Additionally, the influence of (i) the stationary and mobile phases and (ii) the chemical structure of the analytes on retention and resolution was investigated. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
02693879
Volume :
38
Issue :
12
Database :
Complementary Index
Journal :
Biomedical Chromatography
Publication Type :
Academic Journal
Accession number :
180923679
Full Text :
https://doi.org/10.1002/bmc.6020