The Effectivenes of Nephelium mutabile Leaf Extract on Staphylococcus aureus, Escherichia Coli, and Candida Tropicalis Biofilm Formation.

Bacteria such as Staphylococcus aureus and Escherichia coli are the most common causes of foodborne illness. Candida tropicalis is also found in the faeces of chronic diarrhoea patients. Biofilms can form on the surfaces of living or inanimate objects. Biofilm formation contributes to various infections and makes their eradication difficult. The Nephelium mutabile plant is known to have active substances that are antibacterial, anti-oxidative, anti-inflammatory, anti-mutagenic, and anti-carcinogenic. The objective of this study is to determine the effectiveness of Nephelium mutabile leaf extract in inhibiting biofilms formed by Staphylococcus aureus, Escherichia coli, and Candida tropicalis. This study used a Completely Randomized Design [CRD] with two sample groups: the sample group with the addition of Nephelium mutabile extract before biofilm formation and the group sample without any treatment. The concentration of Nephelium mutabileleaf extract for the biofilm inhibition test was 25%. Distilled water is used for negative control. Nephelium mutabile extract was most effective in inhibiting the formation of a Candida tropicalis biofilm [92.24%], and the lowest effectiveness inhibited the formation of Staphylococcus aureus biofilm [35.76%]. Nephelium mutabile leaf extract has the potential to be an antibiofilm for bacteria and fungi [Staphylococcus aureus, Escherichia coli, and Candida tropicalis]. [ABSTRACT FROM AUTHOR]