Back to Search Start Over

Isolation, identification, and mechanism analysis of plant growth-promoting rhizobacteria in tobacco.

Authors :
Chuandong Jiang
Fuyu Peng
Li Zhang
Yuqin Zhang
Jie Wang
Junmin Li
Binghui Cui
Changdai Cao
Chengqiang Wang
Yunlei Qin
Ran Wang
Zongpeng Zhao
Jiazhu Jiang
Mingfeng Yang
Mingming Sun
Long Yang
Qiang Zhang
Source :
Frontiers in Microbiology; 2024, p1-18, 18p
Publication Year :
2024

Abstract

Plant growth, crop yield, and pest and disease control are enhanced by PGPR (Plant growth promoting rhizobacteria), which are beneficial microorganisms found in a close symbiosis with plant roots. Phytohormones are secreted, nutrient uptake is improved, and soil properties along with the microbiological environment are regulated by these microorganisms, making them a significant focus in agricultural research. In this study, the efficient PGPR strain T1 was isolated and screened from tobacco inter-root soil, and identified and confirmed by ITS sequencing technology. Tobacco growth indicators and soil property changes were observed and recorded through potting experiments. The activities of key enzymes (e.g., sucrase, catalase, urease) in soil were further determined. High-throughput sequencing technology was utilized to sequence the soil microbial community, and combined with macro-genomics analysis, the effects of T1 strain on soil microbial diversity and metabolic pathways were explored. Following the application of T1, significant improvements were observed in the height, leaf length, and width of tobacco plants. Furthermore, the physical and chemical properties of the soil were notably enhanced, including a 26.26% increase in phosphorus availability. Additionally, the activities of key soil enzymes such as sucrase, catalase, and urease were significantly increased, indicating improved soil health and fertility. Comprehensive joint microbiomics and macrogenomics analyses revealed a substantial rise in the populations of beneficial soil microorganisms and an enhancement in metabolic pathways, including amino acid metabolism, synthesis, and production of secondary metabolites. These increase in beneficial microorganisms and the enhancement of their metabolic functions are crucial for plant growth and soil fertility. This study provides valuable references for the development of innovative microbial fertilizers and offers programs for the sustainable development of modern agriculture. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1664302X
Database :
Complementary Index
Journal :
Frontiers in Microbiology
Publication Type :
Academic Journal
Accession number :
180663423
Full Text :
https://doi.org/10.3389/fmicb.2024.1457624