Effects of Resistant Starch Supplementation on Metabolic Parameters and Oxidative Stress in C57BL/6 Mice Fed With a High‐Fat Diet.

Normolipid supplemented with resistant starch (RS) is a type of dietary fiber with prebiotic properties, potential action on intestinal permeability, and an effect on decreasing glycemic levels, presenting therapeutic potential for issues stemming from excess body fat and associated comorbidities, such as nonalcoholic fatty liver disease (NAFLD). Sixty C57BL/6 mice were allocated to the following diets for 4 months: control (C), normolipid rich in fiber (F), RS, high fat (HL), HL rich in fiber (HLF), and HL supplemented with RS (HLRS). The following parameters were analyzed: animal weight, body composition, food intake, fasting blood glucose, lipid profile, hepatic and intestinal histology, hepatic and intestinal oxidative stress, and assessment of short‐chain fatty acids in feces. Long‐term supplementation with high doses of RS effectively reduced body weight gain and decreased liver and retroperitoneal adipose tissue weights, blood glucose levels, alanine aminotransferase, and serum triglycerides. Intestinal histology showed that the high‐fat diet increased the total diameter of the intestinal lumen (846.81 ± 7.82) and reduced enteric muscle thickness (8.51 ± 0.51), while RS supplementation effectively improved these parameters (658.01 ± 3.82; 30.56 ± 0.66), also increased butyrate production, and reduced oxidative stress in the intestine. RS supplementation was effective in reducing weight, adiposity, and NAFLD in mice with diet‐induced obesity and metabolic syndrome (MS). [ABSTRACT FROM AUTHOR]