Effect of Functional Bacteria on Petroleum Hydrocarbon Degradation and the Microbial Community Structure.

Based on a polluted site in Tianjin area, the experiment was designed to compare two soil columns in the addition group and the control group by using the screened petroleum hydrocarbon degrading functional bacteria. The effects of the functional bacteria on petroleum hydrocarbon degradation were investigated by measuring the concentrations of petroleum hydrocarbon, HCO3 - and CO3 2-, combining with 16S high-throughput sequencing, comparing the microbial community structure before and after the reaction, and predicting the function of the sequencing results. The results showed that the addition of functional bacteria had a significant effect on the degradation of petroleum hydrocarbons. The species composition of the column showed that the addition of functional bacteria changed the microbial community diversity in the soil column, and the addition of functional bacteria promoted the growth of Desulfobrunum phylum and inhibited the reproduction of Actinobacterium phylum. Desulfoprunum was mainly present at the water outlet of the two groups of experiments, and it was speculated that sulphate redox reactions existed at the water inlet for both groups of experiments. The relative abundance of Thermincola was higher at the outlet of the bacterial addition group, which was accompanied by strong redox reactions such as denitrification and iron reduction. The analyses of alpha diversity showed that the microbial community diversity was higher in the two groups of test outfalls, while the analyses of beta diversity indicated that the microbial community structure may be related to the spatial location of the test columns. In addition, ecological network analysis revealed that Ascomycota was the key phylum in the tests, and the addition of functional bacteria promoted the synergistic and cooperative relationship among species. PICRUSt2 functional prediction analysis showed that the addition of functional bacteria only enhanced the abundance of bamB, bamC in the water intake, and badA, badDEFG in the water outflow. [ABSTRACT FROM AUTHOR]