Recent Studies from Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences Add New Data to Embryonic Stem Cells (Effects of the auxin-dependent degradation of the cohesin and condensin complexes on the...).

Recent studies from the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences focus on the effects of auxin-dependent degradation of cohesin and condensin complexes on the repair of distant DNA double-strand breaks in mouse embryonic stem cells. The research highlights the role of the SMC protein family in maintaining chromosome structure and influencing cellular processes, particularly DNA repair mechanisms. Contrary to expectations, Rad21 depletion did not increase deletion/inversion frequencies, while double condensin depletion showed high biological variability, requiring further investigation. The study utilized Cas9 RNP nucleofection to generate deletions and inversions efficiently and discussed the Neon/ddPCR method for deletion detection in mESCs. [Extracted from the article]