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All-optical reporting of inhibitory receptor driving force in the nervous system.

Authors :
Selfe, Joshua S.
Steyn, Teresa J. S.
Shorer, Eran F.
Burman, Richard J.
Düsterwald, Kira M.
Kraitzick, Ariel Z.
Abdelfattah, Ahmed S.
Schreiter, Eric R.
Newey, Sarah E.
Akerman, Colin J.
Raimondo, Joseph V.
Source :
Nature Communications; 10/19/2024, Vol. 15 Issue 1, p1-18, 18p
Publication Year :
2024

Abstract

Ionic driving forces provide the net electromotive force for ion movement across receptors, channels, and transporters, and are a fundamental property of all cells. In the nervous system, fast synaptic inhibition is mediated by chloride permeable GABA<subscript>A</subscript> and glycine receptors, and single-cell intracellular recordings have been the only method for estimating driving forces across these receptors (DF<subscript>GABAA</subscript>). Here we present a tool for quantifying inhibitory receptor driving force named ORCHID: all-Optical Reporting of CHloride Ion Driving force. We demonstrate ORCHID's ability to provide accurate, high-throughput measurements of resting and dynamic DF<subscript>GABAA</subscript> from genetically targeted cell types over multiple timescales. ORCHID confirms theoretical predictions about the biophysical mechanisms that establish DF<subscript>GABAA</subscript>, reveals differences in DF<subscript>GABAA</subscript> between neurons and astrocytes, and affords the first in vivo measurements of intact DF<subscript>GABAA</subscript>. This work extends our understanding of inhibitory synaptic transmission and demonstrates the potential for all-optical methods to assess ionic driving forces. Single-cell intracellular recordings have been used as the primary tool for estimating driving forces across inhibitory receptors within the nervous system. Here, the authors present ORCHID as an all-optical method to measure inhibitory receptor driving forces in targeted brain cell types. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20411723
Volume :
15
Issue :
1
Database :
Complementary Index
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
180370062
Full Text :
https://doi.org/10.1038/s41467-024-53074-y