Patent Application Titled "Methods of Generating Libraries of Nucleic Acid Sequences for Detection via Flourescent in Situ Sequ" Published Online (USPTO 20240327901).

A patent application titled "Methods of Generating Libraries of Nucleic Acid Sequences for Detection via Fluorescent in Situ Sequ" was published online by inventors from Massachusetts. The application focuses on enhancing hybridization reactions in cells for accurate detection of RNA and DNA sequences using fluorescent in situ sequencing (FISSEQ). The method involves providing a reaction mixture with target nucleic acid molecules, probes, and a hybridization reaction enhancing agent to facilitate the detection process. The patent application outlines various aspects of the method, including the use of polymer backbones, functional groups, enzymatic reactions, and cleavable linkers to improve the efficiency of nucleic acid detection. [Extracted from the article]