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Fundamental Characteristics of Molecularly Imprinted Polymer Nanoparticles with High Affinity for Human Serum Albumin.

Authors :
Youyuan Man
Shoichi Nishitani
Toshiya Sakata
Source :
Sensors & Materials; 2024, Vol. 36 Issue 9, Part 4, p4077-4087, 11p
Publication Year :
2024

Abstract

In this research, the meticulous development and exhaustive analysis of molecularly imprinted polymer nanoparticles (nano-MIPs) are explored, with particular emphasis on their affinity for human serum albumin (HSA), a crucial biomarker for diagnosing type 2 diabetes mellitus. The yield of the synthesized nano-MIPs for HSA was determined by a freeze drying technique to be in the range of 6–15 mg for every 100 g of glass beads. A multifaceted characterization approach, including dynamic light scattering analysis, atomic force microscopy, and Fourier-transform infrared spectroscopy, was employed to rigorously assess the physicochemical properties of the nano-MIPs. The results indicate a relatively uniform size distribution and a well-defined chemical composition. Surface plasmon resonance kinetic analysis further revealed that the nano-MIPs exhibit high affinity and specificity for HSA, with a dissociation constant (Kd) of 2.37 × 10<superscript>−9</superscript> M and an R² value of 0.97. These findings not only validate the successful synthesis and characterization of nano-MIPs but also underscore their potential use for selective molecular recognition, thereby providing promising avenues for future research and applications in biomedical and environmental fields. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09144935
Volume :
36
Issue :
9, Part 4
Database :
Complementary Index
Journal :
Sensors & Materials
Publication Type :
Academic Journal
Accession number :
180260732
Full Text :
https://doi.org/10.18494/SAM4738