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Fundamental Characteristics of Molecularly Imprinted Polymer Nanoparticles with High Affinity for Human Serum Albumin.
- Source :
- Sensors & Materials; 2024, Vol. 36 Issue 9, Part 4, p4077-4087, 11p
- Publication Year :
- 2024
-
Abstract
- In this research, the meticulous development and exhaustive analysis of molecularly imprinted polymer nanoparticles (nano-MIPs) are explored, with particular emphasis on their affinity for human serum albumin (HSA), a crucial biomarker for diagnosing type 2 diabetes mellitus. The yield of the synthesized nano-MIPs for HSA was determined by a freeze drying technique to be in the range of 6–15 mg for every 100 g of glass beads. A multifaceted characterization approach, including dynamic light scattering analysis, atomic force microscopy, and Fourier-transform infrared spectroscopy, was employed to rigorously assess the physicochemical properties of the nano-MIPs. The results indicate a relatively uniform size distribution and a well-defined chemical composition. Surface plasmon resonance kinetic analysis further revealed that the nano-MIPs exhibit high affinity and specificity for HSA, with a dissociation constant (Kd) of 2.37 × 10<superscript>−9</superscript> M and an R² value of 0.97. These findings not only validate the successful synthesis and characterization of nano-MIPs but also underscore their potential use for selective molecular recognition, thereby providing promising avenues for future research and applications in biomedical and environmental fields. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09144935
- Volume :
- 36
- Issue :
- 9, Part 4
- Database :
- Complementary Index
- Journal :
- Sensors & Materials
- Publication Type :
- Academic Journal
- Accession number :
- 180260732
- Full Text :
- https://doi.org/10.18494/SAM4738