Back to Search Start Over

NMR side-chain assignments of the Crimean–Congo hemorrhagic fever virus glycoprotein n cytosolic domain.

Authors :
Brigandat, Louis
Laux, Maëlys
Marteau, Caroline
Cole, Laura
Böckmann, Anja
Lecoq, Lauriane
Fogeron, Marie-Laure
Callon, Morgane
Source :
Magnetic Resonance; 2024, Vol. 5 Issue 2, p95-101, 7p
Publication Year :
2024

Abstract

We assigned the side-chain resonances of the Crimean–Congo hemorrhagic fever virus (CCHFV) glycoprotein n (Gn) cytosolic domain that is 69 amino acids long to complete the backbone resonances previously published by Estrada et al. (2011). The process was facilitated by three factors. First, sample preparation using cell-free protein synthesis (CFPS) was completed in less than 2 d and allowed for correct zinc finger formation by adding zinc ions to the reaction. Second, access to NMR platforms with standardized pulse sequences allowed for data acquisition in 18 d. Third, data analysis using the online platform NMRtist allowed sequential resonance assignments to be made in a day, and assignments were verified and finalized in less than a week. Our work thus provides an example of how NMR assignments, including side chains, of small and well-behaved proteins can be approached in a rapid routine, at protein concentrations of 150 µ M. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
26990016
Volume :
5
Issue :
2
Database :
Complementary Index
Journal :
Magnetic Resonance
Publication Type :
Academic Journal
Accession number :
180239361
Full Text :
https://doi.org/10.5194/mr-5-95-2024