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Mutating the arginine residue within the FRNK motif of telosma mosaic virus (TelMV) HC‐Pro protein attenuates viral infection and confers effective protection against TelMV in passion fruit (Passiflora edulis).

Authors :
Wang, Linxi
Shi, Wei
Aziz, Asma
Wang, Xiaoqing
Liu, Haobin
Shen, Wentao
Cui, Hongguang
Dai, Zhaoji
Source :
Pest Management Science; Oct2024, Vol. 80 Issue 10, p5256-5265, 10p
Publication Year :
2024

Abstract

BACKGROUND: Telosma mosaic virus (TelMV, Potyvirus, Potyviridae) is an emerging viral pathogen that threatens passion fruit plantations worldwide. However, an efficient strategy for controlling such a virus is not yet available. Cross protection is a phenomenon in which pre‐infection of a plant with one mild strain prevents or delays subsequent infection by the same or closely related virus. HC‐Pro is the potyviral encoded multifunctional protein involved in several steps of viral infection, including multiplication, movement, transmission and RNA silencing suppression. In this study, we tested whether it is possible to generate attenuated viral strains capable of conferring protection against severe TelMV infection by manipulating the HC‐Pro gene. RESULTS: By introducing point mutation into the conserved motif FRNK of HC‐Pro that is essential for RNA silencing suppression, we have successfully obtained three attenuated mutants of TelMV (R181K, R181D, and R181E, respectively). These attenuated TelMV mutants could systemically infect passion fruit plants without noticeable symptoms. Pre‐inoculation of one of these attenuated mutants confers efficient protection against subsequent infection by severe TelMV strain. Moreover, we demonstrated that the HC‐Pros harbored by the attenuated mutants exhibit reduced RNA silencing suppression activity in Nicotiana benthamiana leaves. CONCLUSION: The attenuated TelMV mutants developed in this study that are suitable for cross protection offer a practical, powerful tool to fight against TelMV for sustainable passion fruit production. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1526498X
Volume :
80
Issue :
10
Database :
Complementary Index
Journal :
Pest Management Science
Publication Type :
Academic Journal
Accession number :
180231652
Full Text :
https://doi.org/10.1002/ps.8252