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Oxidative stress-modifying effects of TiO2 nanoparticles with varying content of Ti 3+ (Ti 2+) ions.
- Source :
- Nanotechnology; 12/9/2024, Vol. 35 Issue 50, p1-11, 11p
- Publication Year :
- 2024
-
Abstract
- Nanoparticles (NPs) with reactive oxygen species (ROS)-regulating ability have recently attracted great attention as promising agents for nanomedicine. In the present study, we have analyzed the effects of TiO<subscript>2</subscript> defect structure related to the presence of stoichiometric (Ti<superscript>4+</superscript>) and non-stoichiometric (Ti<superscript>3+</superscript> and Ti<superscript>2+</superscript>) titanium ions in the crystal lattice and TiO<subscript>2</subscript> NPs aggregation ability on H<subscript>2</subscript>O<subscript>2</subscript>- and tert-butyl hydroperoxide (tBOOH)-induced ROS production in L929 cells. Synthesized TiO<subscript>2</subscript>-A, TiO<subscript>2</subscript>-B, and TiO<subscript>2</subscript>-C NPs with varying Ti<superscript>3+</superscript>(Ti<superscript>2+</superscript>) content were characterized by x-ray powder diffraction, transmission electron microscopy, small-angle x-ray scattering, x-ray photoelectron spectroscopy, and optical spectroscopy methods. Given the role of ROS-mediated toxicity for metal oxide NPs, L929 cell viability and changes in the intracellular ROS levels in H<subscript>2</subscript>O<subscript>2</subscript>- and tBOOH-treated L929 cells incubated with TiO<subscript>2</subscript> NPs have been evaluated. Our research shows that both the amount of non-stoichiometric Ti<superscript>3+</superscript> and Ti<superscript>2+</superscript> ions in the crystal lattice of TiO<subscript>2</subscript> NPs and NPs aggregative behavior affect their catalytic activity, in particular, H<subscript>2</subscript>O<subscript>2</subscript> decomposition and, consequently, the efficiency of aggravating H<subscript>2</subscript>O<subscript>2</subscript>- and tBOOH-induced oxidative damage to L929 cells. TiO<subscript>2</subscript>-A NPs reveal the strongest H<subscript>2</subscript>O<subscript>2</subscript> decomposition activity aligning with their less pronounced additional effects on H<subscript>2</subscript>O<subscript>2</subscript>-treated L929 cells due to the highest amount of Ti<superscript>3+</superscript>(Ti<superscript>2+</superscript>) ions. TiO<subscript>2</subscript>-C NPs with smaller amounts of Ti<superscript>3+</superscript> ions and a tendency to aggregate in water solutions show lower antioxidant activity and, consequently, some elevation of the level of ROS in H2O2/tBOOH-treated L929 cells. Our findings suggest that synthesized TiO<subscript>2</subscript> NPs capable of enhancing ROS generation at concentrations non-toxic for normal cells, which should be further investigated to assess their possible application in nanomedicine as ROS-regulating pharmaceutical agents. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09574484
- Volume :
- 35
- Issue :
- 50
- Database :
- Complementary Index
- Journal :
- Nanotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 180117310
- Full Text :
- https://doi.org/10.1088/1361-6528/ad7e31