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Sperm quality and in vitro fertilizing ability of boar spermatozoa stored at 4 °C versus conventional storage for 1 week.
- Source :
- Frontiers in Veterinary Science; 9/27/2024, p1-8, 8p
- Publication Year :
- 2024
-
Abstract
- Introduction: Since boar spermatozoa show a marked deterioration in sperm quality when cooled, insemination doses are usually stored at 16-18 °C. However, maintaining this temperature during transport of semen doses is challenging, particularly during the summer months. An alternative could be to store the doses at 4 °C if cold-shock to the sperm could be prevented. The objective of this study was to evaluate boar sperm quality and fertility in in vitro fertilization after storage in AndroStar Premium at 4 °C for 1 week. Methods: Insemination doses (n =9) in AndroStar Premium from a commercial boar semen collection station were transported to the laboratory at approximately 20 °C. At the laboratory, sperm quality evaluation and was preformed and each dose was split; half of each ejaculate was stored in a climate-controlled box at 16-18 °C, the other was slowly cooled to 4 °C. Both samples were stored for 1 week before further sperm quality evaluation and in vitro fertilization (IVF) were performed. Mean values were tested using generalized linear regression, with treatment and boar as fixed factors; p ≤ 0.05 was considered significant. Results: Sperm membrane integrity (mean±sem: 91±0.05 and 83±0.09% for 16 and 4 °C, respectively) and superoxide production (6.79±2.37 and 13.54±6.23% for 16 and 4 °C, respectively), were different between treatments. The DNA fragmentation index was lower in cold-stored samples than in conventionally stored samples (3.74±2.25 and 7.40±3.36% for 4 and 16 °C, respectively). The numbers of oocytes developing to blastocyst on Day 6 (mean±sd: 9.0±8.0 and 6.0±5.0%, for storage at 16 and 4 °C, respectively) were not different between treatments. Discussion: Therefore, storage of boar semen doses in AndroStar Premium at 4 °C for up to 7days would be a viable alternative to current praxis. [ABSTRACT FROM AUTHOR]
- Subjects :
- FERTILIZATION in vitro
ARTIFICIAL insemination
SEMEN
SPERMATOZOA
OVUM
Subjects
Details
- Language :
- English
- ISSN :
- 22971769
- Database :
- Complementary Index
- Journal :
- Frontiers in Veterinary Science
- Publication Type :
- Academic Journal
- Accession number :
- 179992453
- Full Text :
- https://doi.org/10.3389/fvets.2024.1444550