ВЕРИФИЦИРАНЕ НА КОЛИЧЕСТВЕН МЕТОД ЗА ОТКРИВАНЕ И ПРЕБРОЯВАНЕ НА PSEUDOMONAS AERUGINOSA В ПИТЕЙНИ ВОДИ, С ЦЕЛ ПРАВИЛНОТО ПРИЛАГАНЕ НА МЕТОДА И ПОЛУЧАВАНЕ НА ДОСТОВЕРНИ РЕЗУЛТАТИ.

Introduction: Drinking water quality management has been one of the main pillars of primary water prevention for more than a century and a half and continues to be the foundation for the prevention and control of waterrelated diseases. Therefore, the application of verified microbiological methods providing reliable results is of great importance for assessing the quality of drinking water. Aim: Detection and enumeration of Pseudomonas aeruginosa in drinking water experimentally contaminated with a strain of this microorganism. Determining the main characteristics of the membrane filtration method, ensuring the reliability of the results obtained, thus guaranteeing prevention and protecting the consumers health. Material and methods: A standard microbiological method for the detection and enumeration of Pseudomonas aeruginosa in drinking water was applied - BDS EN ISO 16266:2006 „Water quality. Detection and enumeration of Pseudomonas aeruginosa. Membrane filtration method“ as well as standard methods for determining the main characteristics of the method. Twenty samples of 100 ml drinking water were artificially contaminated with CRM Pseudomonas aeruginosa. After incubation of the membrane filters on a selective nutrient medium - Cetrimid agar, confirmatory tests are carried out. Results: The proven Pseudomonas aeruginosa in a given volume of water are all pyocyanin-producing colonies that are oxidase (+), fluoresce under an UV lamp and produce ammonia from acetamide. The data obtained confirming the ability of Pseudomonas aeruginosa as a psychrophile to grow at low temperatures and in nutrient-poor oligotrophic environments, such as drinking water, were used to verify the applied method. Conclusion: The microbiological monitoring of water, through the application of verified microbiological methods, is a basis for preventing infections and a tool for ensuring safe drinking water. The potential risks to consumers can be detected at an early stage and early protection measures can be taken. [ABSTRACT FROM AUTHOR]