The Potential of Hydrolates for use in the Production of Alfalfa Micro Sprouts: Sanitizers and Flavour Enhancers.

Sprouted seeds and microgreens are emerging as innovative specialty raw salad crops, valued for their health-promoting properties. However, the risk of foodborne illnesses associated with microbial contamination in microgreens underscores the need for effective sanitization in their production. This study explores using hydrolates, by-products of essential oil distillation from six plants, as natural sanitizers in alfalfa microgreen production. The research investigates their impact on seed germination, antimicrobial activity, and sensory attributes. Results revealed that oregano, fennel, lavender, and lemon catmint hydrolates decrease alfalfa seed germination, while peppermint and hop hydrolates have no significant impact. Peppermint and fennel hydrolates demonstrate notable efficacy in antimicrobial testing. Sensory analysis indicates differences in odour and flavour, with peppermint, oregano, and lavender receiving high scores. According to the results, peppermint hydrolate can be considered a favourable option for alfalfa micro sprout production, contributing to sustainable and organic approaches in urban agriculture and underlining the importance of natural sanitizers for food safety. Highlights: Micro sprouts are prevalent raw salad crops with functional properties. Alfalfa sprouting was done in water or six selected hydrolates during 10 days. Peppermint, oregano, fennel, hop, lavender and lemon catmint hydrolates were used. Peppermint hydrolate showed the highest antimicrobial and sensory potential. [ABSTRACT FROM AUTHOR]