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Dual-specificity phosphatases 13 and 27 as key switches in muscle stem cell transition from proliferation to differentiation.
- Source :
- Stem Cells; Sep2024, Vol. 42 Issue 9, p830-847, 18p
- Publication Year :
- 2024
-
Abstract
- Muscle regeneration depends on muscle stem cell (MuSC) activity. Myogenic regulatory factors, including myoblast determination protein 1 (MyoD), regulate the fate transition of MuSCs. However, the direct target of MYOD in the process is not completely clear. Using previously established MyoD knock-in (MyoD-KI) mice, we revealed that MyoD targets dual-specificity phosphatase (Dusp) 13 and Dusp27. In Dusp13 : Dusp27 double knock-out mice, the ability for muscle regeneration after injury was reduced. Moreover, single-cell RNA sequencing of MyoD-high expressing MuSCs from MyoD-KI mice revealed that Dusp13 and Dusp27 are expressed only in specific populations within MyoD-high MuSCs, which also express Myogenin. Overexpressing Dusp13 in MuSCs causes premature muscle differentiation. Thus, we propose a model where DUSP13 and DUSP27 contribute to the fate transition of MuSCs from proliferation to differentiation during myogenesis. [ABSTRACT FROM AUTHOR]
- Subjects :
- KNOCKOUT mice
STEM cells
RNA sequencing
MUSCLE cells
PHOSPHATASES
MUSCLE regeneration
Subjects
Details
- Language :
- English
- ISSN :
- 10665099
- Volume :
- 42
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Stem Cells
- Publication Type :
- Academic Journal
- Accession number :
- 179637320
- Full Text :
- https://doi.org/10.1093/stmcls/sxae045