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Detection and molecular characterization of a novel mitovirus associated with Passiflora edulis Sims.

Detection and molecular characterization of a novel mitovirus associated with Passiflora edulis Sims.

Authors :
Santos, Yam Sousa
Vidal, Andreza Henrique
Abreu, Emanuel Felipe Medeiros
Nogueira, Isadora
Faleiro, Fábio Gelape
Lacorte, Cristiano Castro
Melo, Fernando L.
de Araújo Campos, Magnólia
de Rezende, Rafael Reis
Morgan, Tulio
Varsani, Arvind
Alfenas-Zerbini, Poliane
Ribeiro, Simone Graça
Source :
Archives of Virology; Sep2024, Vol. 169 Issue 9, p1-6, 6p
Publication Year :
2024

Abstract

Mitoviruses are cryptic capsidless viruses belonging to the family Mitoviridae that replicate and are maintained in the mitochondria of fungi. Complete mitovirus-like sequences were recently assembled from plant transcriptome data and plant leaf tissue samples. Passion fruit (Passiflora spp.) is an economically important crop for numerous tropical and subtropical countries worldwide, and many virus-induced diseases impact its production. From a large-scale genomic study targeting viruses infecting Passiflora spp. in Brazil, we detected a de novo-assembled contig with similarity to other plant-associated mitoviruses. The contig is ∼2.6 kb long, with a single open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRP). This contig has been named "passion fruit mitovirus-like 1" (PfMv1). An alignment of the predicted amino acid sequence of the RdRP of PfMv1 and those of other plant-associated mitoviruses revealed the presence of the six conserved motifs of mitovirus RdRPs. PfMv1 has 79% coverage and 50.14% identity to Humulus lupulus mitovirus 1. Phylogenetic analysis showed that PfMV1 clustered with other plant-associated mitoviruses in the genus Duamitovirus. Using RT-PCR, we detected a PfMv1-derived fragment, but no corresponding DNA was identified, thus excluding the possibility that this is an endogenized viral-like sequence. This is the first evidence of a replicating mitovirus associated with Passiflora edulis, and it should be classified as a member of a new species, for which we propose the name "Duamitovirus passiflorae". [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03048608
Volume :
169
Issue :
9
Database :
Complementary Index
Journal :
Archives of Virology
Publication Type :
Academic Journal
Accession number :
179407003
Full Text :
https://doi.org/10.1007/s00705-024-06115-2