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Similar usage of T‐cell receptor β‐chain between tumor and adjacent normal tissue in hepatocellular carcinoma.

Authors :
Yang, Jie‐Zuan
Xu, Shao‐Yan
Xiao, Dang‐Sheng
Li, Jin‐You
Jin, Xiu‐Yuan
Yan, Dong
Source :
Cancer Medicine; Aug2024, Vol. 13 Issue 16, p1-10, 10p
Publication Year :
2024

Abstract

Background: In this study, we comprehensively profiled the T‐cell receptor (TCR) repertoire of the tumor and adjacent normal tissue in patients with HBV‐associated hepatocellular carcinoma (HCC) and determined the baseline characteristics and clinical significance of TCR. Methods: High‐throughput sequencing was used to determine the profile of complementarity‐determining region 3 (CDR3) of the TCR‐β chain variable (TRBV) in the tumor and normal tissue samples of 14 HCC patients. At the same time, TRBV diversity and differences in expression between tumor and normal tissues were investigated. The cumulative frequency of top 100 CDR3 (CF100), clonality, and Shannon entropy as indices to evaluate diversity, Results: The diversity of TRBV CDR3 showed no significant difference between tumor and normal tissues. Of the 58 V gene segments in TRBV, TRBV16 and TRBV7‐6 had a significantly higher frequency in the tumor group than in the normal group (p < 0.05). The frequency of 14 J gene segments showed no significant difference between tumor and normal tissues. In contrast, the frequency of 22 TRBVx/BJx combinations was significantly higher in the tumor than in the normal tissue. In addition, the length and type of TRBV CDR3 were similar in tumor and normal tissues, and a Gaussian distribution was observed in both groups. Conclusion: This study provided a large amount of information about the TCR lineage in HBV‐associated HCC, laying the foundation for further research. In addition, the fact that the immune repertoire (TRBV CDR3) hardly differs between tumor and adjacent normal tissue provides a new clue for exploring the mechanism of the liver as an organ with immune privileges. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20457634
Volume :
13
Issue :
16
Database :
Complementary Index
Journal :
Cancer Medicine
Publication Type :
Academic Journal
Accession number :
179393116
Full Text :
https://doi.org/10.1002/cam4.70121